Percentage of calcium in sperm

Looking For Great Deals On Calcium? From Everything To The Very Thing. All On eBay. Get Calcium With Fast And Free Shipping For Many Items On eBay (1990) compared sperm motility, velocity, and progressive movement with total and ionized calcium in patients with normal (>60%) and decreased (<60%) sperm motility. No difference in total calcium was found, but a statistically significant decrease in seminal ionized calcium was found in those men with decreased motility The importance of calcium-dependent sperm processes for fertilization in vitro is well known, but their interaction with sperm transport in vivo is not yet clear. To determine whether exposure to calcium alters sperm physiology after incubation in the uterus, spermatozoa from +/+ mice were incubated in medium with 1.7 mM calcium prior to. ander NJ. Defective calcium influx and acrosome reaction (spontaneous and progesterone induced) in spermatozoa of infertile men with severe teratozoospermia. Fertil Steril 1994;61:249-54. FIGURE 1 Percentage of spermatozoa increasing intracellular calcium in response to P in samples with different sperm quality. (a) Sample types A-E (mean SE) Incubation for 2 or 18h with PGE2 or PGF2α resulted in a significant (P<0.05) increase in the percentage of spermatozoa with progressive motility. In contrast with PGF2α, PGE2 alone induced an increase in sperm intracellular calcium levels; however, the percentage of sperm bound to the human ZP was doubled for both PGs

Protein phosphotyrosine content and the percentage of

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Sperm motility inhibitory factors at this level was proposed as the reason. 54 The level of calcium and magnesium returned back to the level of 1st day of experiment . Supporting reports were available. 21 The results of the present study confirmed that an abstinence to 3-5 days each sufficient to bring back the element to the normal level Percentage of oscillating sperm with calcium values higher than the mean population value with the head oriented towards: the progesterone well (1), the opposite well containing culture medium (3), and up (2) or down (4) the no gradient axes. (TIF Semen is a viscous liquid made up of sperm, aka male reproductive cells, and various other secretions from the male reproductive system that are released during ejaculation A 1992 World Health Organization report described normal human semen as having a volume of 2 mL or greater, pH of 7.2 to 8.0, sperm concentration of 20×10 6 spermatozoa/mL or more, sperm count of 40×10 6 spermatozoa per ejaculate or more, and motility of 50% or more with forward progression (categories a and b) of 25% or more with rapid progression (category a) within 60 minutes of ejaculation

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  1. al fluid; the constituents of its secretions are mainly citric acid, acid phosphatase, calcium, sodium, zinc, potassium, protein-splitting enzymes, and fibrolysin (an enzyme that reduces blood and tissue fibres)
  2. Mechanisms. Mammalian sperm cells become more active when they approach an egg cell in a process called sperm activation.Sperm activation has been shown to be caused by calcium ionophores in vitro, progesterone released by nearby cumulus cells and binding to ZP3 of the zona pellucida.. The initial change is called hyperactivation, which causes a change in spermatozoa motility
  3. al plasma calcium and magnesium. Total sperm motility deteriorated to 50% in 8 h whereas active and progressive type reduced to 50% in 4 h
  4. Sperm from European eel males treated with hCGrec was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, SW) and in the absence of calcium (NaCl+EDTA), and treated with calcium inhibitors or modulators. The sperm motility parameters were evaluated by
  5. However, buffering of intracellular calcium levels with the calcium chelator BAPTA-AM resulted in an increase in polyspermy. Together, these data suggest that a postfertilization decrease in egg membrane receptivity to sperm requires functions of the egg actin cytoskeleton that are disrupted by cytochalasin D

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8, 12 and 24 h time for percentage of (1) total sperm motility, (2) active and progressive and (3) non-progressive motility and seminal plasma calcium and magnesium. Total sperm motility deteri It is generally accepted that the higher the calcium ionophore dose, the lower the percentage of motile sperm and the higher the percentage of the acrosome-reacted sperm. The drop in motility under the influence of calcium ionophore may be connected, as suggested by Fraser & Dermott [1992] , with the action of Ca 2+ ATP-ase

Sixty percent of calcium entry was inhibited by methoxy-verapamil, suggesting that activation, of voltage dependent calcium channels (VDCC) may be involved in the process. A significant correlation (R2 = 0.88, p<0.006) was found between the percentage of capacitated spermatozoa and the [CaZ+]i increase Rosmarinus of ficinalis leaves (AEROL), calcium chloride (CaCl 2) and their combinations to Tris extender on post-cr yopr eserved sperm's cell individual motility and live sperm percentage for low-semen quality of Holstein bulls for different preservation periods (cooling at 5 0C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC). The. Effect of GnRH on sperm-zona pellucida binding in the presence or absence of extracellular calcium. Sperm capacitated for 4.5 h were treated for 5 min with saline (control) or with 75 nM GnRH in the presence (+Ca 2+) or absence (−Ca 2+) of 2.5 mM CaCl 2 before the hemizona assay. The results are expressed as a percentage (mean ± SEM) of the control hemizonae, and they represent the average.

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Sperm from European eel males treated with hCG rec was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, SW) and in the absence of calcium (NaCl + EDTA), and treated with calcium inhibitors or modulators. The sperm motility parameters were evaluated by a computer-assisted sperm analysis (CASA) system, and changes in the [Ca 2 +] i fluorescence. To reach and fertilize an egg, sperm must move — wriggling and swimming through a woman's cervix, uterus and fallopian tubes. This is known as motility. You're most likely to be fertile if at least 40% of your sperm are moving. Structure (morphology). Normal sperm have oval heads and long tails, which work together to propel them In contrast, DGC-sperm showed non-significant differences between time zero and 4 hours incubations for percentage of cells showing calcium oscillations, frequency and amplitude, while SU-sperm exhibited an increase in the percentage of cells showing calcium oscillations and frequency after capacitation for 4 hours

Samples of the testes and sperm cells were analyzed by cytochemical techniques to determine the location of calcium and Ca 2+ ‐ATPase and the percentage of acrosome reactions under light and electron microscopy. The data showed that (1) Sertoli cells exhibited numerous calcium precipitates as large, round, electron‐dense bodies distributed. Water accounts for what percentage of the human body's weight? 60%. What is the most abundant mineral in soft water? What trace mineral is essential to sperm production and taste perception, and assists in immune function? zinc. Which statement about calcium metabolism is true? When the body needs more calcium, proteins in the intestinal. However, in the bovine species sperm viability remains unaffected, while in equine sperm, the percentage of viable sperm was significantly reduced . These results seem to be species-specific as calcium has an inhibitory effect on equine sperm capacitation [26] but not in bovine sperm [27] The researchers found that 13, or 45 percent, of the 29 UV filters tested induced calcium ion influxes in the sperm cells, thus interfering with normal sperm cell function. This effect began at very low doses of the chemicals, below the levels of some UV filters found in people after whole-body application of sunscreens, Skakkebaek said Penetration of the zona pellucida allows the sperm and oocyte cell membranes to fuse almost immediately. Calcium enters the oocyte and the sperm cell stop moving. The rise in intracellular calcium results in 3 changes in the oocyte: The egg cell membrane depolarises in order to prevent polyspermy - this is the primary block

Zinc, magnesium and calcium in human seminal fluid

Sperm volume 4.3 ml Ph 8.3 Sperm density 15.66 X 10 ^6 ml semen Sperm progression 26% Sperm count 67×10^6 %non motile 67% Liquifaction time 6 minutes Morphology 7% Sara SDx says: August 28, 2017 at 5:20 p Sperm comes from the testicles and accounts for ~2-5% of all semen. While this may not seem like a lot, there are 20 - 100 million little spermies per mL of ejaculate. And because men produce anywhere from 2 - 6 mL of semen, some can shoot out 600 million spermatozoa in one single ejaculation

There needs to be enough sperm in the semen. If the number of sperm in your semen (sperm count) is low, it decreases the odds that one of your sperm will fertilize your partner's egg. A low sperm count is fewer than 15 million sperm per milliliter of semen or fewer than 39 million per ejaculate. Sperm must be functional and able to move The acrosome reaction was induced in ram, rabbit, and human sperm by incubating with the ionophore A23187 plus calcium ions, although the percentage of reacted sperm was different for each species.

For example, in some mammals, an increase in pH and calcium ions activates sperm. The ultimate effect is membrane hyperpolarization which activates the sperm. Figure 1. The image above shows the detailed structure of a human sperm cell. Evaluating Sperm Motility. The percentage of motile sperm is the most widely used measurement of semen quality Sperm Intracellular Calcium Levels and Acrosome Membrane Integrity Are Not Affected by TER-Induced JNK Phosphorylation. The relative Fluo3 fluorescence intensity (Fluo3 +) of the viable sperm population (PI -) is presented in Figure 9, whereas the percentage of viable sperm with an intact acrosome (PNA - /PI -) is shown in Figure 10

Calcium alters capacitation and progressive motility of

If you're behind a web filter, please make sure that the domains *.kastatic.org and *.kasandbox.org are unblocked After selection, various sperm parameters were studied, such as sperm recovery rate, sperm morphology, motility (by CASA), vitality, acrosome status and intracellular calcium (by flow cytometry) and ability to penetrate oocytes in vitro FemGlide caused a significant decrease in sperm motility, as well as an increase in the percentage of sperm with DNA damage. Agarwal et al, 2007: Aquagel (water-based) Aquagel appears to reduce sperm motility by about half after 10 minutes of exposure to semen. Mackenzie et al, 2019: Sylk (water-based Therefore, we estimated intracellular calcium in post-thaw sperm using fluorescent dye Fluo-3 AM by flow cytometry and found that 10 µM RU 486 treated sperm had more percent of live sperm with.

Determination of human sperm calcium uptake evaluating

  1. after A 23187 treatment (10
  2. The researchers found that 13, or 45 percent, of the 29 UV filters tested induced calcium ion influxes in the sperm cells, thus interfering with normal sperm cell function
  3. To test the effect of calcium on individual sperm, a calcium-free suspension of sperm models was placed in a slide chamber, which was constructed of a coverslip resting on two strips of silicone grease. After settling, the unattached sperm were flushed from the chamber with fresh buffer solution without calcium, but with 0.5 mM EGTA
  4. Sperm motility analysis was carried out on both rat and porcine sperm (suspended in TBM) treated with calcium and with increasing doses (range 1-1000 nM) of calcimimetic AMG 641 (Amgen, Thousand Oaks, California). Sperm on TCM (with and without AMG 641) was also used as a positive control for sperm motility (Bravo et al, 2005)
  5. al calcium level and motility characteristics (the percentage of sperm motility and sperm grade activity). A significant negative correlation was found between the se
  6. The in vivo concentration of bicarbonate (HCO3−), one of the essential sperm capacitating effectors, varies greatly in the different environments sperm go through from cauda epididymis to the fertilisation site. On the contrary, porcine in vitro sperm capacitation and fertilisation media usually contains a standard concentration of 25 mmol/L, and one of the main problems presented is the.

The acrosome reaction was induced in ram, rabbit, and human sperm by incubating with the ionophore A23187 plus calcium ions, although the percentage of reacted sperm was different for each species: 90% in ram, 38% in rabbit, and 69% in man However, while sperm activation in a Ca-free media (NaCI + EDTA) resulted in a percentage of motility similar to seawater, the [Ca2+](i) levels did not increase at all. This result strongly suggests that increasing [Ca2+](i) is not a pre-requisite for the induction of sperm motility in European eel sperm Data from three independent experiments were accumulated. Percentage shed sperm is indicated. (E) GM1 status with calcium ionophore treatment. Number of sperm examined: A23187−, n=308; A23187+, n=313. Data from three independent experiments were accumulated. Percentage entire head sperm is indicated

Low physiological levels of prostaglandins E2 and F2α

Although a significant percentage of sperm show a decrease in [Na +] i, another fraction maintains it at high levels. These observations warrant future investigations on whether different capacitation-associated changes could occur in the same sperm population. Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3. In another study, Vanden Meerschaut et al. found that the percentage of oocytes injected with wobbler sperm showed less significant rises in calcium, lower fertilization rate, and lower development to the blastocyst stage compared with the wild type Calcium 200 mg, 15 percent DV ; Magnesium 50 mg, 12 percent DV ; Phosphorus 20 mg, 2 percent DV; Potassium 80 mg, 2 percent DV; Chloride 72 mg, 3 percent DV ; Iodine 150 mcg, 100 percent DV ; Iron 18 mg, 100 percent DV ; Zinc 11 mg, 100 percent DV; Selenium 55 mcg, 100 percent DV ; Manganese 2.3 mg, 100 percent DV ; Copper 0.5 mg, 56 percent D Sperm motility is a key parameter in selecting sperm for in vitro fertilization procedures. Motile sperm has a lower frequency of DNA damage. Günter Wagner , the Alison Richard Professor of Ecology and Evolutionary Biology at Yale, and Jamie Maziarz , a research associate in Wagner's lab on Yale's West Campus, are co-authors of the study

Video: Effect of daily ejaculation on semen quality and calcium

Versatile Action of Picomolar Gradients of Progesterone on

Semen Nutrition: What's in Semen? Does It Have Nutritional

Sperm concentration (also called sperm count or sperm density)—this is measured in millions of sperm per milliliter of semen. Normal is at least 20 million or more sperm per milliliter, with a total ejaculate volume of 80 million or more sperm. Fewer sperm and/or a lower sperm concentration may impair fertility Sperm activation has been shown to be caused by calcium ionophores in vitro, progesterone released by nearby cumulus cells and binding to ZP3 of the zona pellucida. The cumulus cells are embedded in a gel-like substance made primarily of hyaluronic acid, and developed in the ovary with the egg and support it as it grows

calcium increase, indicating progesterone and ATP can modulate calcium channel Table S 1 . Percentage of sperm responding to progesterone and also to the inhibitors . Fig 2B no. of experiment Total sperm Responding sperm % of responding sperm Sp + P4 4 84 33 39.28571429 dp + P4 4 81 38 46.91358025 Fig 3 Calories in Sperm based on the calories, fat, protein, carbs and other nutrition information submitted for Sperm The role of the sperm factors in the oocyte activation. In the normal activation process, the acrosome reaction (1) allows the exposure of PT for the fusion of the sperm membrane with the oocyte (2), starting in the equatorial segment and continuing to the PAS-PT, until penetration (3). SOAF are released into the ooplasm and triggers the Ca + 2 oscillations via IP 3

Semen - Wikipedi

A study in infertile men showed that taking 1,000-mg vitamin C supplements twice a day for up to 2 months increased sperm motility by 92% and sperm count by more than 100%. It also reduced the. Male sperm is comprised of vitamins, minerals and proteins. The capacity of male sperm is to help in the multiplication of the human species. Taking premature ejaculation pills doesn't work to increase protein in male sperm to any degree. So, do not start abusing them with that thought in mind 0.375, 0.75, 1.5, and 3 µg/mL on sperm motility and viability as well asat 3 µg/mL on NO production and calcium chelation in semen were assessed. Lansoprazole at 3 µg/mL significantly decreased total and progressive sperm motility (P = 0.0021, P = 0.0256, respectively), but not sperm viability (P = 0.8763). I This maintenance of blood calcium levels is an example of _____. When blood calcium levels are high, calcium is removed from your blood and deposited in your bones. When blood calcium levels are low, an appropriate physiological response would be to _____. a.) thermoregulation; lower the need for calcium in nerves and muscle

Calcium in biology - Wikipedia

A defective sperm that lacks DNA can produce a spurious calcium spike. In the lab, scientists can coax unfertilized eggs into beginning the post-fertilization process by simply injecting them with. What's in sperm? Will swallowing make my girlfriend gain weight? There are many ingredients composing semen, and its makeup is the same from man to man Yet in that 1 percent may be found 150 million sperm, 150 million human aspirants yearning to meet their mammoth other halves. a telltale shift in calcium ions. The sperm become. The motility, intracellular concentration of free Ca2+ and DNA integrity of sperm with or without terahertz (0.1 to 3 THz) irradiation at 60 µW/cm2 were assessed. We found that terahertz irradiation for more than 5 minutes significantly increased the progressive motility percentage of sperm, and the DNA integrity was not changed The mechanisms involved in the regulation of mammalian sperm motility are not well understood. Calcium ions (Ca2+) have been suggested to play a key role in the maintenance of motility; nevertheless, how Ca2+ modulates this process has not yet been completely characterized. Ca2+ can bind to calmodulin and this complex regulates the activity of multiple enzymes, including Ca2+/calmodulin.

semen Definition, Characteristics, & Production Britannic

Hyperactivation - Wikipedi

Effect of Extracellular Ca 2+ on Sperm Motility. The microscopical assay revealed that application of external Ca 2+ to calcium-free medium promotes sperm motility. The percentage of forward motility increased up to 20 % when the external Ca 2+ concentration was 10 µM (Fig. 1).Above this concentration Ca 2+ showed an inhibitory effect, as forward motility gradually decreased (Fig. 1) It has been shown that the sperm plasma membrane after cryopreservation is injured and capacitation and/or acrosome reaction is enhanced due to elevated intracellular calcium levels of frozen-thawed sperm (Maxwell & Johnson, Reference Maxwell and Johnson 1997; Bailey et al., Reference Bailey, Bilodeau and Cormier 2000) The only way to determine this is to have a semen analysis with measurement of the number of sperm, the sperm motility (movement) and the amount of normal sperm (percentage). If the semen analysis shows a reasonable sperm count, motility and percentage of normal sperm forms, it is wise to have the sperm banked (frozen) for potential. As shown in Fig. 7, the presence of extracellular calcium at concentration in normal seawater (11 mmol l −1) did not affect sperm migration. In the presence of the high-voltage-activated (HVA) Ca 2+ channel blocker nifedipine (50 μmol l −1 ) ( Stork and Cocks, 1994 ), spermatozoa migration was similar to that observed in the presence of. Zinc is an essential mineral involved in numerous enzymes. It plays a role in antioxidant enzymes, brain function, and the immune system, among many other biological roles. Zinc is most commonly taken to reduce the frequency of illness and to support optimal levels of testosterone

Time bound changes (in 24 h) in human sperm motility and

  1. One process, the acrosomal reaction, is the fusion of the acrosomal vesicle and the sperm plasma membrane, which results in the extension of the acrosomal process. The acrosomal reaction is initiated by a fucose-containing polysaccharide within the egg jelly, which binds to the sperm and allows calcium to enter the sperm head
  2. The sperm plasma membrane and outer acrosomal membrane have increased permeability and fluidity as a result of these changes. The more permeable sperm plasma membrane allows for influx of calcium and bicarbonate resulting in activation of second messengers and initiation of signaling events
  3. D helped control calcium levels, which is essential for sperm motility. Alvarez recommends 15 mcg of vita
  4. The intact and uncapacitated percentage of sperm was higher in prefreeze-processed than the control at 8 h incubation time. The drastic reduction of proportion uncapacitated sperm in control at 8 h is an evidence that more sperm which were uncapacitated at first but may have partially damaged plasma membrane will demonstrate cryocapacitation.
  5. Whereas Lgals1 ‐/‐ and WT sperm showed comparable percentages of hairpin cells at high osmolarity conditions, Lgals1 ‐/‐ spermatozoa exhibited a percentage of angulated tails significantly higher than WT sperm when incubated in the lower osmolyte content medium (Fig. 5B), suggesting that Gal‐1 deficiency impairs the ability of sperm.
  6. Coral reefs cover less than 1 percent of the ocean floor — all the reefs combined would equal an area of about 110,000 square miles (285,000 square km), only about the size of the state of Nevada

The most common features are palatal differences (~75 percent), heart defects (75 percent), problems fighting infection (77 percent), low calcium levels (50 percent), differences in the kidney (35 percent), characteristic facial appearance (numbers vary depending on the individual's ethnic and racial background), learning problems (~90 percent. CatSper is a sperm specific voltage-sensitive calcium channel, which is mainly located at the flagellar part of the sperm (9). Increase in intracellular pH is a main activator of CatSper channel (9), and it has been shown that alkalization of bovine sperm by [NH.sub.4]Cl causes its hyperactivation via opening of CatSper channel (10)

Role of calcium on the initiation of sperm motility in the

However, increased intrasperm concentration of calcium ions was determined to correlate negatively with sperm viability (Kumar et al., 2008). Hypertension is a global health concern which affects all races, sexes and different age groups; though it is more prevalent in the adults S. S. Suarez, S. M. Varosi, and X. Dai, Intracellular calcium increases with hyperactivation in intact, moving hamster sperm and oscillates with the flagellar beat cycle, Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 10, pp. 4660-4664, 1993 3.1.2. Sperm Head Vacuoles, Classical Parameters of Sperm Quality, and Male Factors. Our data show that sperm head vacuoles were present in both the normal and abnormal sperm samples (Figure 1).Interestingly, there was a relatively high proportion (74.6%) of spermatozoa with sperm head vacuoles in the sperm of normal quality, thus indicating that the sperm head vacuoles are also found in. New tools to study sperm ionic currents, and lecular cloning of the calcium sensing receptor (CaSR) es-tablished its role as a first messenger (61). To function in Ion channels in general constitute a very minor percentage of the total membrane protein. In this regard, demonstrat Sperm whale, (Physeter catodon), also called cachalot, the largest of the toothed whales, easily recognized by its enormous square head and narrow lower jaw.The sperm whale is dark blue-gray or brownish, with white patches on the belly. It is thickset and has small paddlelike flippers and a series of rounded humps on its back. Males attain a maximum length of about 24 metres (78.7 feet) and.

Other calcium-rich vegetables include okra, kale, cabbage, broccoli, and Brussels sprouts. This gives about 3-6% of RDI per 1/2 cup (60-80 grams). That means that vegetables also contain different levels of drugs, such as oxalates. Oxalates can bind calcium in your stomach, making it more difficult for your body to absorb Although an overall percentage is impossible to calculate due to varying specific gravities of semen and protein content, it is close to 50% protein by weight (although no volume). As the higher end of average ejaculate volume is 10mL for human men, [3] this is approximately 0.5g (500mg) of dietary protein per ejaculate on average A sperm cell gets its propulsive power from a long and powerful tail, and while there's still much to be learned about how that tail functions, scientists do know that the calcium within the. reservation bank is effective in maintaining acceptable reproductive potential and motility of sperm during cryopreservation. This study aimed to evaluate the antioxidant effects of different doses of the extract of brown algae Sargassum on oxidative stress and frozen human sperm parameters. We conducted a randomized controlled trial on the semen samples from 11 healthy men in the age group of.

Sperm cells up close. So what do we know about sperm cells? There are certainly a lot of them. A fertile male usually churns out between 5 million to 250 million sperm cells per milliliter of ejaculate (the average human ejaculate is between 0.75 mL and 2.5 mL) Atorvastatin calcium is a white to off-white crystalline powder that is insoluble in aqueous solutions of pH 4and below. Atorvastatin calcium is very slightly soluble in distilled water, pH 7.4 phosphate buffer, and acetonitrile; slightly soluble in ethanol; and freely soluble in methanol.. LIPITOR Tablets for oral administration contain 10, 20, 40, or 80 mg of atorvastatin and the following. Progress 09/01/02 to 08/31/05 Outputs Bovine oocyte activation: Characterization of calcium responses by intracytoplasmic sperm injection and role of IP3R-1 in fertilization. Objective 1: To determine if sperm delivered by ICSI can initiate fertilization-like oscillations in bovine oocytes. Objective 2: To investigate if capacitation, acrosome reaction, or fertilization enhance the ability of. Calcium, as an intracellular messenger, and vitamin D, as a steroid Ca2+ regulatory hormone, play vital roles in regulation of cellular processes. The purpose of this research project is to determine cellular and molecular mechanisms by which Ca2+ regulates growth and development of skeletal muscle and mammary gland, sperm and mammary functions, and cellular responses to stress in domestic. 0.1 milligram riboflavin (4 percent DV) 37.4 milligrams calcium (4 percent DV) 2016, investigated the effect of cadmium on alterations in sperm count and activity and found that the tested methanol extract of breadfruit caused significant improvement in sperm count, motility (movement) and hormone levels..

Schematic diagram of the apparatus used for the extractionTransient exposure to calcium ionophore enables in vitroIn vitro effects of cationic compounds on functional human(PDF) Induction of the acrosome reaction in dog sperm
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